{"id":3523,"date":"2021-10-07T12:53:27","date_gmt":"2021-10-07T10:53:27","guid":{"rendered":"https:\/\/equipes.lps.u-psud.fr\/sobio\/?page_id=3523"},"modified":"2025-08-22T18:34:30","modified_gmt":"2025-08-22T16:34:30","slug":"time-resolved-saxs","status":"publish","type":"page","link":"https:\/\/equipes.lps.u-psud.fr\/sobio\/time-resolved-saxs\/","title":{"rendered":"Time-resolved SAXS"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Small-angle X-ray scattering (SAXS) has become a powerful technique over the last decades for studying biological macromolecules in solution with a nanometer-scaled resolution. The system of interest can be studied in its native conditions and need not be ordered as is the case for X-ray crystallography. Thanks to the development of high brilliance synchrotron sources and to the considerable progress realized on detectors, time-resolved SAXS (TR-SAXS) gives now access to dynamical processes occurring over a few milliseconds.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"181\" src=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2025\/08\/synchrotrons.png\" alt=\"\" class=\"wp-image-3929\" srcset=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2025\/08\/synchrotrons.png 500w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2025\/08\/synchrotrons-300x109.png 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><figcaption class=\"wp-element-caption\">(<em>Left) Synchrotron SOLEIL near Orsay. (Right) European Synchrotron Radiation Facility (ESRF) in Grenoble.<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><br>We develop algorithms that process the large amount of data generated by TR-SAXS experiments. For example, by constructing kinetic models that account for the self-assembly and disassembly of icosahedral viral capsids, we have identified the structure of long-lived intermediate species as well as the evolution of their concentration, consistently with spatio-temporal scattering measurements.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"280\" src=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2025\/08\/algorithm_tr-saxs.png\" alt=\"\" class=\"wp-image-3931\" srcset=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2025\/08\/algorithm_tr-saxs.png 500w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2025\/08\/algorithm_tr-saxs-300x168.png 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><figcaption class=\"wp-element-caption\"><em>Workflow for analysis and modeling of TR-SAXS data.<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><br>Preliminary kinetic data can be obtained in-house with our static light scattering setup coupled to a stopped-flow apparatus. The latter is a SFM-4000 model from Biologic equipped with four syringes and a hard-stop valve. Rapid mixing can be achieved in a few milliseconds. The scattered light intensity is collected at 90\u00b0 with a photomultiplier tube. Time-resolved fluorescence and absorbance measurements can also be carried out at wavelengths comprised between 300 and 700 nm.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"257\" src=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2025\/08\/stopped-flow.jpg\" alt=\"\" class=\"wp-image-3933\" srcset=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2025\/08\/stopped-flow.jpg 500w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2025\/08\/stopped-flow-300x154.jpg 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/figure>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-7387b849 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><\/div>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Related publications<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>M.\u00a0CHEVREUIL, D. LAW-HINE, J. CHEN, S. BRESSANELLI, S. COMBET, D. CONSTANTIN, J. DEGROUARD, J. M\u00d6LLER, M.\u00a0ZEGHAL, G. TRESSET (2018) Nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging genome or polyelectrolyte. <em>Nat. Commun.<\/em> <strong>9<\/strong> 3071.<\/li>\n\n\n\n<li>D. LAW-HINE, M.\u00a0ZEGHAL, S. BRESSANELLI, D. CONSTANTIN, G. TRESSET (2016) Identification of a major intermediate along the self-assembly pathway of an icosahedral viral capsid by using an analytical model of a spherical patch. <em>Soft Matter <\/em><strong>12<\/strong> 6728-6736.<\/li>\n\n\n\n<li>D. LAW-HINE, A. K. SAHOO, V. BAILLEUX, M.\u00a0ZEGHAL, S. PREVOST, P. K. MAITI, S. BRESSANELLI, D. CONSTANTIN, G. TRESSET (2015) Reconstruction of the disassembly pathway of an icosahedral viral capsid and shape determination of two successive intermediates. <em>J. Phys. Chem. Lett.<\/em> <strong>6<\/strong> 3471-3476.<\/li>\n\n\n\n<li>G. TRESSET, C. LE COEUR, J.-F. BRYCHE, M.\u00a0TATOU, M.\u00a0ZEGHAL, A. CHARPILIENNE, D. PONCET, D. CONSTANTIN, S. BRESSANELLI (2013) Norovirus capsid proteins self-assemble through biphasic kinetics via long-lived stave-like intermediates. <em>J. Am. Chem. Soc.<\/em> <strong>135<\/strong> 15373-15381.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Contact<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"mailto:guillaume.tresset@universite-paris-saclay.fr\" target=\"_blank\" rel=\"noreferrer noopener\">Guillaume Tresset<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Small-angle X-ray scattering (SAXS) has become a powerful technique over the last decades for studying biological macromolecules in solution with a nanometer-scaled resolution. The system of interest can be studied &#8230;<\/p>\n","protected":false},"author":21,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3523","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages\/3523","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/users\/21"}],"replies":[{"embeddable":true,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/comments?post=3523"}],"version-history":[{"count":7,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages\/3523\/revisions"}],"predecessor-version":[{"id":3934,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages\/3523\/revisions\/3934"}],"wp:attachment":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/media?parent=3523"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}