{"id":3375,"date":"2021-10-05T21:13:15","date_gmt":"2021-10-05T19:13:15","guid":{"rendered":"https:\/\/equipes.lps.u-psud.fr\/sobio\/?page_id=3375"},"modified":"2026-02-11T19:40:41","modified_gmt":"2026-02-11T18:40:41","slug":"cryo-electron-microscopy","status":"publish","type":"page","link":"https:\/\/equipes.lps.u-psud.fr\/sobio\/cryo-electron-microscopy\/","title":{"rendered":"Cryo electron microscopy"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Cryo electron microscopy (cryo-EM) let us visualize biological and soft matter objects at (sub) nanometer scale while preserving their hydrated state and native ionic environment, vitrified at low temperature. We access their conformations and interactions and are able to explore conformational changes, in the aim to relate these to their functional activities.<br><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">We use both thin film cryo-EM, to image macromolecular complexes and nano-objects (viruses, nanoparticles, liposomes,\u2026) in solution, and CEMOVIS (Cryo-Electron Microscopy Of VItreous Sections) to image biological tissue and cells, or bulk liquid crystals and polymers.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"336\" src=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-20-a\u0300-20.56.09-1024x336.png\" alt=\"\" class=\"wp-image-3376\" style=\"width:740px;height:242px\" srcset=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-20-a\u0300-20.56.09-1024x336.png 1024w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-20-a\u0300-20.56.09-300x98.png 300w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-20-a\u0300-20.56.09-768x252.png 768w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-20-a\u0300-20.56.09-1536x503.png 1536w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-20-a\u0300-20.56.09.png 1764w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"908\" height=\"346\" src=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/image.png\" alt=\"\" class=\"wp-image-3379\" srcset=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/image.png 908w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/image-300x114.png 300w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/image-768x293.png 768w\" sizes=\"auto, (max-width: 908px) 100vw, 908px\" \/><figcaption class=\"wp-element-caption\"><strong>A few examples of thin film cryo-EM at LPS.<\/strong> (A) Viromimetic particles. (B) Cubosome (phytantriol\/polymer\/water). (C) Gold nanoparticle supra-crystals. (D, E) DNA toroids confined in bacteriophage (T5, Lambda) capsids. (F) Imogolite nanotubes. (G) Nano-emulsion stabilized by colloidal nanoparticles. (H) DNA fragments condensed by protamines.<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"908\" height=\"422\" src=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/image-1.png\" alt=\"\" class=\"wp-image-3380\" srcset=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/image-1.png 908w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/image-1-300x139.png 300w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/image-1-768x357.png 768w\" sizes=\"auto, (max-width: 908px) 100vw, 908px\" \/><figcaption class=\"wp-element-caption\"><strong>Cryo electron microscopy of vitreous sections. <\/strong>From left to right, top to bottom: basal body of<em>Paramecium tetraurelia<\/em>; thylakoid membranes of <em>Euglena gracilis<\/em>; intracellular crystalline inclusion; lamello-columnar phase of nucleosomes in solution; two views of Mushroom Bodies in <em>Drosophila <\/em>brain.<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Equipments<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Cryo electron microscope JEOL 2010F: 200kV with field emission gun and <em>CRYO<\/em> pole piece, equipped with <em>Hole Free Phase Plates<\/em> and Direct Electron Detector (K2 summit, Gatan)<\/li>\n\n\n\n<li>Cryo-holders (Gatan 626) and cryo-transfer stations<\/li>\n\n\n\n<li>Thin film vitrification robots: Vitrobot Mark IV (Thermofisher) &amp; homemade robot optimized for visquous specimens<\/li>\n\n\n\n<li>Liquid helium slam-freezing device for bulk specimen vitrification (Cryovacublock, Reichert)<\/li>\n\n\n\n<li>Cryo-ultramicrotome (Leica UC6\/FC6) in controlled environment (T, RH), equipped with a micro-manipulator and fluorescence microscope<\/li>\n\n\n\n<li>Sputter\/coater (Leica ACE)<\/li>\n\n\n\n<li>Plasma cleaner (Cressington)<\/li>\n\n\n\n<li>Liquid cell holder Poseidon (Protochips)<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"294\" src=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-22-a\u0300-16.44.22-1024x294.png\" alt=\"\" class=\"wp-image-3377\" srcset=\"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-22-a\u0300-16.44.22-1024x294.png 1024w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-22-a\u0300-16.44.22-300x86.png 300w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-22-a\u0300-16.44.22-768x221.png 768w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-22-a\u0300-16.44.22-1536x441.png 1536w, https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-content\/uploads\/sites\/10\/2021\/10\/Capture-de\u0301cran-2021-09-22-a\u0300-16.44.22-2048x588.png 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Access<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">The LPS cryo-EM platform is a plug-in-lab Paris-Saclay (<a href=\"https:\/\/www.pluginlabs-universiteparissaclay.fr\/fr\/results\/keywords\/text\/CRYOTEM\">CRYOTEM@LPS<\/a>) and is part of the national facility <a href=\"https:\/\/metsa.ows.fr\/\">METSA<\/a>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Contacts<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Am\u00e9lie Leforestier&nbsp;: amelie.leforestier@universite-paris-saclay.fr<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Fatima Taiki : fatima.taiki@universite-paris-saclay.fr<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cryo electron microscopy (cryo-EM) let us visualize biological and soft matter objects at (sub) nanometer scale while preserving their hydrated state and native ionic environment, vitrified at low temperature. We &#8230;<\/p>\n","protected":false},"author":21,"featured_media":3383,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3375","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages\/3375","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/users\/21"}],"replies":[{"embeddable":true,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/comments?post=3375"}],"version-history":[{"count":6,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages\/3375\/revisions"}],"predecessor-version":[{"id":4089,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages\/3375\/revisions\/4089"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/media\/3383"}],"wp:attachment":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/media?parent=3375"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}