{"id":3021,"date":"2021-05-31T16:10:36","date_gmt":"2021-05-31T14:10:36","guid":{"rendered":"https:\/\/vmtest.lps.u-psud.fr\/testequipe\/?p=3021"},"modified":"2026-04-03T15:09:21","modified_gmt":"2026-04-03T13:09:21","slug":"biochemistry-molecular-biology","status":"publish","type":"page","link":"https:\/\/equipes.lps.u-psud.fr\/sobio\/biochemistry-molecular-biology\/","title":{"rendered":"Biochemistry and molecular biology"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Biological samples (proteins, DNA of defined length, nucleosomes, chromatin fragments, bacteriophages etc.) are prepared in the labororatory using classical methods in biochemistry and molecular biology.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.equipes.lps.u-psud.fr\/sobio\/squelettes-dist\/puce.gif\" alt=\"-\" width=\"8\" height=\"11\">&nbsp; Over-expression of recombinant DNA and proteins (<em>E. coli<\/em>), extraction and purification<br><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.equipes.lps.u-psud.fr\/sobio\/squelettes-dist\/puce.gif\" alt=\"-\" width=\"8\" height=\"11\">&nbsp; Cloning and bacteria transformation (PCR, DNA engineering)<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Our lab is equipped with all experimental devices required for cell and bacterial culture, recombinant protein ingineering, DNA and protein purification:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>HPLC<\/li><li>standard gel electrophoresis (agarose, acrylamide)<\/li><li>preparative gel electrophoresis (Prep cell Bio-Rad, FPLC)<\/li><li>incubators<\/li><li>sterile glove box and hoods<\/li><li>4 centrifuges and 1 ultracentrifuge<\/li><li>PCR<\/li><li>1 cold room (4\u00b0C, 6 m2)<\/li><li>2 deep freezers (-80\u00b0C)<\/li><li>Nanodrop<\/li><li>Spectrophotometre UV-VIS<\/li><\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Contacts<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Laetitia Poncet\u00a0: laetitia.poncet@universite-paris-saclay.fr<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Biological samples (proteins, DNA of defined length, nucleosomes, chromatin fragments, bacteriophages etc.) are prepared in the labororatory using classical methods in biochemistry and molecular biology. &nbsp; Over-expression of recombinant DNA &#8230;<\/p>\n","protected":false},"author":1,"featured_media":3403,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3021","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages\/3021","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/comments?post=3021"}],"version-history":[{"count":6,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages\/3021\/revisions"}],"predecessor-version":[{"id":3408,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/pages\/3021\/revisions\/3408"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/media\/3403"}],"wp:attachment":[{"href":"https:\/\/equipes.lps.u-psud.fr\/sobio\/wp-json\/wp\/v2\/media?parent=3021"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}