CNRS research director
Université Paris-Saclay

Biography

My research aims to address fundamental issues in self-organizing biological matter at the microscopic scale using experimental and theoretical concepts derived from soft condensed matter physics. In particular, I focus on the high-resolution structure of ordered and weakly disordered systems, as well as on the nonequilibrium dynamics of assembly and self-organization phenomena. I exploit state-of-the-art techniques including cryotransmission electron microscopy and time-resolved radiation scattering with large-scale facilies, and develop models that account for the observed complex behaviors.

Interests

  • Biological self-assembly and self-organization
  • Viruses
  • DNA condensates
  • Small-angle scattering
  • Microfluidics

Education

  • Accreditation to supervise research (2013), Université Paris-Sud
  • PhD in physics (2002), Université Aix-Marseille I
  • MSc. in physics (1998), Université Paris-Sud
  • MEng. in electrical engineering (1998), Ecole Supérieure d’Electricité (Supélec)

Statistical data analysis

Master 2 Systèmes Biologiques et Concepts Physiques
Doctoral school EDPIF Physique en Ile-de-France

Five or ten 3-hours sessions each split into half lecture and half practicals in Python.

Research in physics requires the acquisition, analysis and interpretation of experimental measurements in order to verify an hypothesis and/or devise a theoretical model. The great variability inherent to many physical systems makes it essential to take into account the uncertainties and to use powerful statistical tools available today via Python. Each session consists of a first theoretical part that presents the fundamental concepts of statistical data analysis, illustrated in the second part of the course by practical work in Python through concrete situations encountered in physics and biophysics. The main topics covered include a reminder of probabilities and distributions, statistical tests (chi2, Student, p-value), handling experimental uncertainties (confidence intervals), linear and non-linear adjustments (least squares, covariance matrix, correlations), and some advanced notions (bayesian inference, statistical learning).

Small-angle X-ray scattering and colloidal systems

Ecole Supérieure de Physique et de Chimie Industrielle de la Ville de Paris (ESPCI)

Two sessions of 2 hours each.

This course is an introduction to small-angle X-ray scattering applied to dilute and crystallized colloidal systems. It gives the fundamentals of X-ray scattering by one electron, then the interferences obtained in the presence of two electrons and the generalization with a continuous electron density. The main results for a solution of spherical particles are deduced analytically. Actual scattering data of colloidal suspensions are analyzed thus providing the methodology for characterizing nanoparticles and liquid crystals at the microscopic scale.

Virus self-assembly in complex environments

Viruses are amazing biological agents in which hundreds of molecular blocks integrate with atomic precision into the final structure. Their regularity is all the more remarkable because for many viruses it occurs spontaneously, in an efficient self-assembly process, whether in the host cell or in a test tube. Many viruses are assembled in a crowded intracellular environment, with a low error rate, despite numerous nonspecific interactions with cellular components. Our objective is to decipher the self-assembly pathways of icosahedral viruses in complex, cell-like environments, by setting up a cross-disciplinary strategy that combines experimental physics and quantitative biology with an advanced soft-matter theoretical framework.


Out-of-equilibrium cellular droplets

Living cells are organized into distinct subcompartments to facilitate spatiotemporal regulation of biological reactions. While this organization is traditionally thought to rely on membrane-delimited organelles, such as the nucleus and mitochondria, it also involves membrane-less assemblies of proteins and nucleic acids that are kept together only by weak, dynamical interactions between their components. In the last years groundbreaking studies suggested that these cellular assemblies have liquid-like physical properties and are formed via liquid-liquid phase separation (LLPS) processes. We plan to rely on state-of-the-art techniques in soft matter physics and on an advanced molecular modeling framework for unveiling general features of cellular LLPS in equilibrium and nonequilibrium conditions
and linking them back to their molecular origin.


Marangoni flows

The Marangoni effect is a spectacular manifestation of the interfacial forces driven by surfactants at the air-water interface. It stems from the gradients of surface tension due to the inhomogeneity of the surfactant density across the interface, which subsequently induce the
motion of water over a thin layer. This project aims at elucidating the flows generated in the bulk by visualizing them with a fluorescent probe.

Showing results 1 to 63 of 63 total
  • Conference papers

    Thomas Bugea, Roméo Suss, Siyu Li, Laetitia Gargowitsch Poncet, Charles Truong, et al.. Self-assembly dynamics of simple icosahedral nucleocapsids under high and low capsid protein concentrations. Spanish Network in Physical Virology, Mar 2025, Miraflores de la Sierra (Province de Madrid), Spain. ⟨hal-05003574⟩

    Year of publication

  • Poster communications

    Thomas Bugea, Roméo Suss, Laetitia Gargowitsch Poncet, Charles Truong, Karen Perronet, et al.. Probing single-molecule dynamics in self-assembling viral nucleocapsids. Biophysical Society Annual Meeting, Feb 2025, Los angeles, CA, United States. Biophysical Journal, 124 (3), pp.54a. ⟨hal-04987623⟩

    Year of publication

    Available with open access

  • Journal articles

    Thomas Bugea, Roméo Suss, Laetitia Gargowitsch, Charles Truong, Karen Perronet, et al.. Probing Single-Molecule Dynamics in Self-Assembling Viral Nucleocapsids. Nano Letters, 2024, 24 (46), pp.14821-14828. ⟨10.1021/acs.nanolett.4c04458⟩. ⟨hal-04771478⟩

    Year of publication

    Available with open access

  • Conference papers

    Tamizhmalar Sundararajan, Sandrine Mariot, Theyencheri Narayanan, Guillaume Tresset. Phase separation kinetics of bio-inspired condensates under passive and active conditions. Journées de la Matière Condensée, Oct 2024, Marseille, France. ⟨hal-04794955⟩

    Year of publication

    Available with open access

  • Conference papers

    Thomas Bugea, Suss Roméo, Laetitia Gargowitsch Poncet, Guillaume Tresset, Karen Perronet. Visualizing the self-assembly mechanism of viruses through fluorescence microscopy and interferometric scattering at the single molecule level. PhysChemCell2023, Oct 2024, Orsay, France. ⟨hal-04740989⟩

    Year of publication

  • Poster communications

    Suss Roméo, Thomas Bugea, Laetitia Gargowitsch Poncet, Charles Truong, Karen Perronet, et al.. Visualising the self-assembly process of icosahedral viruses through fluorescence microscopy at single particle level. PhysChemCell2024, Oct 2024, Orsay, France. ⟨hal-04741012⟩

    Year of publication

  • Journal articles

    Guillaume Tresset, Siyu Li, Laetitia Gargowitsch, Lauren Matthews, Javier Pérez, et al.. Glass-like Relaxation Dynamics during the Disorder-to-Order Transition of Viral Nucleocapsids. Journal of Physical Chemistry Letters, 2024, 15 (40), pp.10210-10218. ⟨10.1021/acs.jpclett.4c02158⟩. ⟨hal-04739187⟩

    Year of publication

  • Conference papers

    Charles Truong, Thomas Bugea, Baptiste Bouhet, Guillaume Tresset, François Marquier, et al.. Photobleaching Step Counting and Localization for Fluorescence Microscopy. 2024 46th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), Jul 2024, Orlando (Floride), United States. pp.1-4, ⟨10.1109/EMBC53108.2024.10782704⟩. ⟨hal-04869324⟩

    Year of publication

    Available with open access

  • Poster communications

    Tamizhmalar Sundararajan, Sandrine Mariot, Theyencheri Narayanan, Guillaume Tresset. Phase separation kinetics of bio-inspired condensates under passive and active conditions. 7th International Conference on Physics and Biological Systems, Jun 2024, Palaiseau, France. ⟨hal-04662680⟩

    Year of publication

  • Poster communications

    Renata Naporano Bicev, Louise E. A. Souza, Ana B. Caliari, Ly Porosk, Ülo Langel, et al.. Cell-Penetrating Peptides from the SARS-CoV-2 Proteome: Structural Insights and Therapeutic Potential. 7th International Conference on Physics and Biological Systems, Jun 2024, Palaiseau, France. ⟨hal-04662700⟩

    Year of publication

  • Conference papers

    Thomas Bugea, Karen Perronet, Guillaume Tresset. Visualising the self-assembly dynamics of icosahedral viruses through fluorescence microscopy at the single particle level. Physics and Biological Systems 2024, Jun 2024, Palaiseau (Ecole Polytechnique), France. ⟨hal-04638524⟩

    Year of publication

  • Poster communications

    Tamizhmalar Sundararajan, Sandrine Mariot, Theyencheri Narayanan, Guillaume Tresset. Phase separation kinetics of bioinspired condensates in passive and active conditions. EMBL Symposium: Cellular mechanisms driven by phase separation, May 2024, Heidelberg, Germany. ⟨hal-04590465⟩

    Year of publication

  • Conference papers

    Guillaume Tresset. Glass-like relaxation dynamics during the disorder-order transition of viral nucleocapsids. APS March Meeting, Mar 2024, Minneapolis (MN), United States. ⟨hal-04590274⟩

    Year of publication

  • Poster communications

    Thomas Bugea, Laetitia Gargowitsch, Charles Truong, Karen Perronet, Guillaume Tresset. Visualising the self-assembly dynamics of icosahedral viruses through fluorescence microscopy at the single-particle level. 68th Biophysical Society Annual Meeting, Feb 2024, Philadelphia (PA), United States. Biophysical Journal, 123 (3), pp.224a, 2024, ⟨10.1016/j.bpj.2023.11.1425⟩. ⟨hal-04590348⟩

    Year of publication

  • Journal articles

    Ana Caliari, Renata Bicev, Caroline da Silva, Sinval de Souza, Marta da Silva, et al.. Self-assembly, cytocompatibility, and interactions of desmopressin with sodium polystyrene sulfonate. Soft Matter, 2024, 20 (48), pp.9597-9613. ⟨10.1039/D4SM01125B⟩. ⟨hal-04850610⟩

    Year of publication

  • Conference papers

    Guillaume Tresset. Antivirals alter the kinetic assembly pathway and elastic properties of Hepatitis B virus capsids. ICMS Workshop on Non-Equilibrium Physics of Self-Assembly: From Viruses to Nano-Containers, Nov 2023, Edimbourg (Ecosse), United Kingdom. ⟨hal-04590311⟩

    Year of publication

  • Journal articles

    Kalouna Kra, Siyu Li, Laetitia Gargowitsch Poncet, Jéril Degrouard, Javier Pérez, et al.. Energetics and Kinetic Assembly Pathways of Hepatitis B Virus Capsids in the Presence of Antivirals. ACS Nano, 2023, 17 (13), pp.12723-12733. ⟨10.1021/acsnano.3c03595⟩. ⟨hal-04160101⟩

    Year of publication

    Available with open access

  • Conference papers

    Kalouna Kra, Siyu Li, Laetitia Gargowitsch Poncet, Jéril Degrouard, Javier Pérez, et al.. Energetics and kinetic assembly pathways of Hepatitis B virus in the presence of antivirals. PSB Symposium Dynamics in Structural Biology, Jul 2023, Grenoble, France. ⟨hal-04265846⟩

    Year of publication

  • Conference papers

    Guillaume Tresset. Viruses as bioinspired nanocapsules. 3rd International Conference on Bioengineering and Polymer Science, Jun 2023, Bucarest, Romania. ⟨hal-04265857⟩

    Year of publication

  • Conference papers

    Thomas Bugea, Guillaume Tresset, Karen Perronet. SINGLEVIRUS: Visualising the self-assembly dynamics of icosahedral viruses through fluorescence microscopy at the single particle level. Journée de la communauté des nanosciences et nanotechnologies de Paris-Saclay - Édition 2023, Feb 2023, Orsay, France. ⟨hal-04241174⟩

    Year of publication

  • Poster communications

    Thomas Bugea, Laetitia Gargowitsch Poncet, Charles Truong, Karen Perronet, Guillaume Tresset. Visualising the self-assembly process of icosahedral viruses through fluorescence microscopy at single particle level. Physical Virology Gordon Research Conference, Jan 2023, Lucca, Italy. ⟨hal-04151009⟩

    Year of publication

  • Journal articles

    Haiqin Du, Sandra Kalem, Cécile Huin, Nicolas Illy, Guillaume Tresset, et al.. Engineering of ion permeable planar membranes and polymersomes based on β-cyclodextrin-cored star copolymers. Journal of Colloid and Interface Science, 2023, 630, Part A, pp.465-476. ⟨10.1016/j.jcis.2022.09.147⟩. ⟨hal-04265767⟩

    Year of publication

    Available with open access

  • Journal articles

    Renata Naporano Bicev, Maximilia Frazão de Souza Degenhardt, Cristiano Luis Pinto de Oliveira, Emerson Rodrigo da Silva, Jéril Degrouard, et al.. Glucose restriction in Saccharomyces cerevisiae modulates the phosphorylation pattern of the 20S proteasome and increases its activity. Scientific Reports, 2023, 13 (1), pp.19383. ⟨10.1038/s41598-023-46614-x⟩. ⟨hal-04497985⟩

    Year of publication

    Available with open access

  • Journal articles

    Gwendoline Delecourt, Laetitia Plet, Yann Le Guen, Ozgul Tezgel, Guillaume Tresset, et al.. Synthesis of Double Hydrophilic Block Copolymers Poly(2‐isopropyl‐2‐oxazoline‐ b ‐ethylenimine) and their DNA Transfection Efficiency. Macromolecular Bioscience, 2022, 23 (1), pp.2200296. ⟨10.1002/mabi.202200296⟩. ⟨hal-04265776⟩

    Year of publication

    Available with open access

  • Journal articles

    Cesar Usma, Sandrine Mariot, Claire Goldmann, Matthieu Roché, Anniina Salonen, et al.. Fluorescent Marangoni Flows under Quasi-Steady Conditions. Langmuir, 2022, 38 (30), pp.9129-9135. ⟨10.1021/acs.langmuir.2c00619⟩. ⟨hal-03855203⟩

    Year of publication

    Available with open access

  • Poster communications

    Thomas Bugea, Laetitia Gargowitsch Poncet, Karen Perronet, Guillaume Tresset. Visualizing the self-assembly process of icosahedral viruses through fluorescence microscopy at single molecule level. Physics and Biological Systems 2022, Jun 2022, Gif sur Yvette, France. ⟨hal-03711875⟩

    Year of publication

  • Journal articles

    Milosz Ruszkowski, Aleksander Strugala, Paulina Indyka, Guillaume Tresset, Marek Figlerowicz, et al.. Cryo-EM reconstructions of BMV-derived virus-like particles reveal assembly defects in the icosahedral lattice structure. Nanoscale, 2022, 14 (8), pp.3224-3233. ⟨10.1039/D1NR05650F⟩. ⟨hal-03607847⟩

    Year of publication

    Available with open access

  • Conference papers

    Kalouna Kra, Laetitia Gargowitsch, Stéphane Bressanelli, Guillaume Tresset. Influence of assembly modulators on the assembly kinetics and structure of hepatitis B virus capsid. Biophysical Society Annual Meeting, Feb 2022, San Francisco, United States. pp.167a, ⟨10.1016/j.bpj.2021.11.1889⟩. ⟨hal-03607876⟩

    Year of publication

  • Conference papers

    Laurent Marichal, Laetitia Gargowitsch, Rafael Leite Rubim, Christina Sizun, Kalouna Kra, et al.. Relationships between RNA topology and nucleocapsid structure in a model icosahedral virus. Biophysical Society Annual Meeting, Feb 2022, San Francisco, United States. pp.330a-331a, ⟨10.1016/j.bpj.2021.11.1107⟩. ⟨hal-03607880⟩

    Year of publication

    Available with open access

  • Conference papers

    Kalouna Kra, Maelenn Chevreuil, Laetitia Gargowitsch Poncet, Lauriane Lecoq, Shishan Wang, et al.. Self-assembly kinetic pathways of hepatitis B virus capsids in the presence or not of antivirals. XVIII International Small-Angle Scattering Conference, Sep 2022, Campinas, Brazil. ⟨hal-04265821⟩

    Year of publication

  • Poster communications

    Laurent Marichal, Jean Philippe Renault, Jeril Degrouard, Rafael Leite Rubim, Guillaume Tresset, et al.. Biophysical study of large macromolecular complexes. Physbio2021 - 5th International Conference on Physics & Biological Systems, Jun 2021, E-Conference, France. . ⟨cea-03356115⟩

    Year of publication

    Available with open access

  • Journal articles

    Laurent Marichal, Laetitia Gargowitsch, Rafael Leite Rubim, Christina Sizun, Kalouna Kra, et al.. Relationships between RNA Topology and Nucleocapsid Structure in a Model Icosahedral Virus. Biophysical Journal, 2021, 120 (18), pp.3925-3936. ⟨10.1016/j.bpj.2021.08.021⟩. ⟨hal-03351285⟩

    Year of publication

    Available with open access

  • Journal articles

    Maelenn Chevreuil, Lauriane Lecoq, Shishan Wang, Laetitia Gargowitsch, Naïma Nhiri, et al.. Nonsymmetrical Dynamics of the HBV Capsid Assembly and Disassembly Evidenced by Their Transient Species. Journal of Physical Chemistry B, 2020, 124 (45), pp.9987-9995. ⟨10.1021/acs.jpcb.0c05024⟩. ⟨hal-03665643⟩

    Year of publication

    Available with open access

  • Journal articles

    Maelenn Chevreuil, Lauriane Lecoq, Shishan Wang, Laetitia Gargowitsch, Naïma Nhiri, et al.. Nonsymmetrical Dynamics of the HBV Capsid Assembly and Disassembly Evidenced by Their Transient Species. Journal of Physical Chemistry B, 2020, 124, pp.9987 - 9995. ⟨10.1021/acs.jpcb.0c05024⟩. ⟨hal-03009814⟩

    Year of publication

    Available with open access

  • Journal articles

    Ming Ni, Guillaume Tresset, Ciprian Iliescu, Charlotte Hauser. Ultrashort Peptide Theranostic Nanoparticles by Microfluidic-Assisted Rapid Solvent Exchange. IEEE Transactions on NanoBioscience, 2020, 19 (4), pp.627-632. ⟨10.1109/TNB.2020.3007103⟩. ⟨hal-03035736⟩

    Year of publication

    Available with open access

  • Conference papers

    Laurent Marichal, Rafael Leite Rubim, Sanaz Panahandeh, Siyu Li, Stéphane Bressanelli, et al.. Transition désordre-ordre et sélection du génome dans un virus icosaédrique modèle à ARN simple brin. XXIIe Journées Francophones de Virologie, Apr 2020, Paris, France. ⟨hal-03046033⟩

    Year of publication

    Available with open access

  • Journal articles

    Sanaz Panahandeh, Siyu Li, Laurent Marichal, Rafael Leite Rubim, Guillaume Tresset, et al.. How a Virus Circumvents Energy Barriers to Form Symmetric Shells. ACS Nano, 2020, 14 (3), pp.3170-3180. ⟨10.1021/acsnano.9b08354⟩. ⟨hal-03035711⟩

    Year of publication

    Available with open access

  • Journal articles

    Haiqin Du, Fernando de Oliveira, Lindomar Albuquerque, Guillaume Tresset, Ewa Pavlova, et al.. Polyglycidol-Stabilized Nanoparticles as a Promising Alternative to Nanoparticle PEGylation: Polymer Synthesis and Protein Fouling Considerations. Langmuir, 2020, 36 (5), pp.1266-1278. ⟨10.1021/acs.langmuir.9b03687⟩. ⟨hal-03035730⟩

    Year of publication

    Available with open access

  • Conference papers

    Maelenn Chevreuil, Sonia Fieulaine, Laetitia Poncet, Karen Perronet, Thomas Zinn, et al.. Characterization of the Assembly and Disassembly of Capsid Proteins Derived from Hepatitis B Virus. 63rd Annual Meeting of the Biophysical Society, Mar 2019, Baltimore, United States. ⟨hal-02076428⟩

    Year of publication

  • Journal articles

    Jean-Pierre Gomez, Guillaume Tresset, Chantal Pichon, Patrick Midoux. Improved histidinylated lPEI polyplexes for skeletal muscle cells transfection. International Journal of Pharmaceutics, 2019, 559, pp.58-67. ⟨10.1016/j.ijpharm.2019.01.003⟩. ⟨hal-02067297⟩

    Year of publication

    Available with open access

  • Journal articles

    Nicolas Illy, Vincent Corcé, Jeremy M Zimbron, Vincent Molinié, Mélanie Labourel, et al.. pH‐Sensitive Poly(ethylene glycol)/Poly(ethoxyethyl glycidyl ether) Block Copolymers: Synthesis, Characterization, Encapsulation, and Delivery of a Hydrophobic Drug. Macromolecular Chemistry and Physics, 2019, 220 (16), pp.1900210. ⟨10.1002/macp.201900210⟩. ⟨hal-02282220⟩

    Year of publication

    Available with open access

  • Conference papers

    Guillaume Tresset. Impact of RNA on the assembly dynamics of icosahedral and tubular viruses. Physical Virology Gordon Research Conference, 2019, Ventura (CA), United States. ⟨hal-04939533⟩

    Year of publication

  • Journal articles

    Maelenn Chevreuil, Didier Law-Hine, Jingzhi Chen, Stéphane Bressanelli, Sophie Combet, et al.. Nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging genome or polyelectrolyte. Nature Communications, 2018, 9, pp.3071. ⟨10.1038/s41467-018-05426-8⟩. ⟨hal-02345735⟩

    Year of publication

    Available with open access

  • Journal articles

    Andrew Burke, Maelenn Chevreuil, Alisier Paris, Vanessa Delagrange, Claire Goldmann, et al.. Nanoparticle-Templated Self-Assembly of Viral Capsids Probed by Time-Resolved Absorbance Spectroscopy and X-Ray Scattering. Physical Review Applied, 2018, 10 (5), ⟨10.1103/PhysRevApplied.10.054065⟩. ⟨hal-02348488⟩

    Year of publication

    Available with open access

  • Journal articles

    Jingzhi Chen, Yves Lansac, Guillaume Tresset. Interactions between the Molecular Components of the Cowpea Chlorotic Mottle Virus Investigated by Molecular Dynamics Simulations. Journal of Physical Chemistry B, 2018, 122 (41), pp.9490-9498. ⟨10.1021/acs.jpcb.8b08026⟩. ⟨hal-02123581⟩

    Year of publication

  • Conference papers

    Maelenn Chevreuil, Karen Perronet, Michael Nassal, Stéphane Bressanelli, Guillaume Tresset. Single-molecule monitoring of Hepatitis B Virus nucleocapsid assembly. XXe Journées Francophones de Virologie, Mar 2018, Paris, France. ⟨hal-01781714⟩

    Year of publication

  • Journal articles

    Ming Ni, Guillaume Tresset, Ciprian Iliescu. Self-assembled polysulfone nanoparticles using microfluidic chip. Sensors and Actuators B: Chemical, 2017, 252, pp.458-462. ⟨10.1016/j.snb.2017.06.028⟩. ⟨hal-04882283⟩

    Year of publication

  • Journal articles

    Jingzhi Chen, Maelenn Chevreuil, Sophie Combet, Yves Lansac, Guillaume Tresset. Investigating the thermal dissociation of viral capsid by lattice model. Journal of Physics: Condensed Matter, 2017, 29 (47), ⟨10.1088/1361-648X/aa8d88⟩. ⟨hal-04882330⟩

    Year of publication

    Available with open access

  • Journal articles

    Guillaume Tresset, Jingzhi Chen, Maelenn Chevreuil, Naima Nhiri, Eric Jacquet, et al.. Two-Dimensional Phase Transition of Viral Capsid Gives Insights into Subunit Interactions. Physical Review Applied, 2017, 7 (1), ⟨10.1103/PhysRevApplied.7.014005⟩. ⟨hal-02140237⟩

    Year of publication

  • Journal articles

    Didier Law-Hine, Mehdi Zeghal, Stéphane Bressanelli, Doru Constantin, Guillaume Tresset. Identification of a major intermediate along the self-assembly pathway of an icosahedral viral capsid by using an analytical model of a spherical patch.. Soft Matter, 2016, 12 (32), pp.6728-36. ⟨hal-01356007⟩

    Year of publication

  • Journal articles

    Didier Law-Hine, Anil K Sahoo, Virginie Bailleux, Mehdi Zeghal, Sylvain Prevost, et al.. Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates.. Journal of Physical Chemistry Letters, 2015, 6 (17), pp.3471-6. ⟨10.1021/acs.jpclett.5b01478⟩. ⟨hal-01446050⟩

    Year of publication

  • Journal articles

    Ciprian Iliescu, Guolin Xu, Wen Hao Tong, Fang Yu, Cătălin Mihai Bălan, et al.. Cell patterning using a dielectrophoretic–hydrodynamic trap. Microfluidics and Nanofluidics, 2015, 19 (2), pp.363-373. ⟨10.1007/s10404-015-1568-2⟩. ⟨hal-04882654⟩

    Year of publication

  • Journal articles

    Ciprian Iliescu, Guillaume Tresset. Microfluidics-Driven Strategy for Size-Controlled DNA Compaction by Slow Diffusion through Water Stream. Chemistry of Materials, 2015, 27 (24), pp.8193-8197. ⟨10.1021/acs.chemmater.5b04129⟩. ⟨hal-04882672⟩

    Year of publication

  • Journal articles

    Benoit Maury, Cristine Gonçalves, Guillaume Tresset, Mehdi Zeghal, Hervé Cheradame, et al.. Influence of pDNA availability on transfection efficiency of polyplexes in non-proliferative cells.. Biomaterials, 2014, 35 (22), pp.5977-5985. ⟨10.1016/j.biomaterials.2014.04.007⟩. ⟨hal-01179393⟩

    Year of publication

  • Journal articles

    Ciprian Iliescu, Cətəlin Mərculescu, Shrinivas Venkataraman, Baptiste Languille, Hanry Yu, et al.. On-chip controlled surfactant-DNA coil-globule transition by rapid solvent exchange using hydrodynamic flow focusing. Langmuir, 2014, 30 (44), pp.13125-13136. ⟨10.1021/la5035382⟩. ⟨hal-04882767⟩

    Year of publication

  • Journal articles

    Guillaume Tresset, Mouna Tatou, Clémence Le Cœur, Mehdi Zeghal, Virginie Bailleux, et al.. Weighing polyelectrolytes packaged in viruslike particles. Physical Review Letters, 2014, 113 (12), ⟨10.1103/PhysRevLett.113.128305⟩. ⟨hal-04882750⟩

    Year of publication

  • Journal articles

    Guillaume Tresset, Clémence Le Coeur, Jean-François Bryche, Mouna Tatou, Mehdi Zeghal, et al.. Norovirus Capsid Proteins Self-Assemble through Biphasic Kinetics via Long-Lived Stave-like Intermediates. Journal of the American Chemical Society, 2013, 135 (41), pp.15378-15381. ⟨10.1021/ja403550f⟩. ⟨hal-00880969⟩

    Year of publication

  • Habilitation à diriger des recherches

    Guillaume Tresset. Compartimentation microscopique: depuis les microchambres femtolitriques jusqu'aux particules pseudo-virales. Matière Molle [cond-mat.soft]. Université Paris Sud - Paris XI, 2013. ⟨tel-00848365⟩

    Year of publication

    Available with open access

  • Journal articles

    Guillaume Tresset, Catalin Marculescu, Anniina Salonen, Ming Ni, Ciprian Iliescu. Fine control over the size of surfactant-polyelectrolyte nanoparticles by hydrodynamic flow focusing. Analytical Chemistry, 2013, 85 (12), pp.5850-5856. ⟨10.1021/ac4006155⟩. ⟨hal-04882940⟩

    Year of publication

  • Journal articles

    Guillaume Tresset, Yves Lansac, Guillaume Romet ‐ Lemonne. Supramolecular Assemblies of Lipid-Coated Polyelectrolytes. Langmuir, 2012, 28 (13), pp.5743--5752. ⟨10.1021/la2048135⟩. ⟨hal-01716459⟩

    Year of publication

  • Journal articles

    Guillaume Tresset, Yves Lansac. Long-range architecture of single lipid-based complex nanoparticles with local hexagonal packing. Journal of Physical Chemistry Letters, 2011, 2 (1), pp.41-46. ⟨10.1021/jz101430e⟩. ⟨hal-04883291⟩

    Year of publication

  • Journal articles

    Ciprian Iliescu, Guillaume Tresset, Guolin Xu. Dielectrophoretic field-flow method for separating particle populations in a chip with asymmetric electrodes. Biomicrofluidics, 2009, 3 (4), ⟨10.1063/1.3251125⟩. ⟨hal-04896727⟩

    Year of publication

    Available with open access

  • Journal articles

    Guillaume Tresset. Generalized Poisson-Fermi formalism for investigating size correlation effects with multiple ions. Physical Review E : Statistical, Nonlinear, and Soft Matter Physics, 2008, 78 (6), ⟨10.1103/PhysRevE.78.061506⟩. ⟨hal-04896845⟩

    Year of publication

Tresset, G. (2018). Genome- and Polymer-filled Icosahedral Viruses. Journées de la Diffusion Neutronique, Roquebrune-sur-Argens (France).
Tresset, G. (2018). Understanding Virus Self-Assembly from X-ray and Neutron Scattering. 7th Spring World Congress on Engineering and Technology, Guilin (Chine).
Tresset, G. (2024, March 4). Glass-like relaxation dynamics during the disorder-order transition of viral nucleocapsids [Conférence]. American Physical Society March Meeting, Minneapolis (USA).
Tresset, G. (2018). Chemins Cinétiques d’Assemblage de Capsides Virales Icosaédriques Empaquetant du Génome à ARN [Conférence]. XXèmes Journées Francophones de Virologie, Paris , France.
Tresset, G. (2023). Energetics and kinetic assembly pathways of Hepatitis B virus in the presence of antivirals [Conférence]. PSB Symposium Dynamics in Structural Biology, Grenoble, France.
Tresset, G. (2023, November). Antivirals alter the kinetic assembly pathway and elastic properties of Hepatitis B virus capsids [Conférence]. ICMS Workshop on Non-Equilibrium Physics of Self-Assembly: From Viruses to Nano-Containers, Edinburgh (UK).
Kra, K., Li, S., Gargowitsch, L., Degrouard, J., Pérez, J., Zandi, R., Bressanelli, S., & Tresset, G. (2023). Energetics and Kinetic Assembly Pathways of Hepatitis B Virus Capsids in the Presence of Antivirals. ACS Nano, 17(13), 12723–12733. https://doi.org/10.1021/acsnano.3c03595
Tresset, G. (2023, January 6). Viruses as bioinspired nanocapsules. 3rd International Conference on Bioengineering and Polymer Science, Bucarest (Roumanie).
Delecourt, G., Plet, L., Guen, Y. L., Tezgel, O., Tresset, G., Midoux, P., Montier, T., Bennevault, V., & Guégan, P. (2022). Synthesis of Double Hydrophilic Block Copolymers Poly(2‐isopropyl‐2‐oxazoline‐ b ‐ethylenimine) and their DNA Transfection Efficiency. Macromolecular Bioscience, 2200296. https://doi.org/10.1002/mabi.202200296
Usma, C. L., Mariot, S., Goldmann, C., Roché, M., Salonen, A., & Tresset, G. (2022). Fluorescent Marangoni Flows under Quasi-Steady Conditions. Langmuir, 38(30), 9129–9135. https://doi.org/10.1021/acs.langmuir.2c00619
Ruszkowski, M., Strugala, A., Indyka, P., Tresset, G., Figlerowicz, M., & Urbanowicz, A. (2022). Cryo-EM reconstructions of BMV-derived virus-like particles reveal assembly defects in the icosahedral lattice structure. Nanoscale, 14(8), 3224–3233. https://doi.org/10.1039/D1NR05650F
Marichal, L., Gargowitsch, L., Rubim, R. L., Sizun, C., Kra, K., Bressanelli, S., Dong, Y., Panahandeh, S., Zandi, R., & Tresset, G. (2021). Relationships between RNA topology and nucleocapsid structure in a model icosahedral virus. Biophysical Journal, 120(18), 3925–3936. https://doi.org/10.1016/j.bpj.2021.08.021
Du, H., de Oliveira, F. A., Albuquerque, L. J. C., Tresset, G., Pavlova, E., Huin, C., Guégan, P., & Giacomelli, F. C. (2020). Polyglycidol-Stabilized Nanoparticles as a Promising Alternative to Nanoparticle PEGylation: Polymer Synthesis and Protein Fouling Considerations. Langmuir, 36(5), 1266–1278. https://doi.org/10.1021/acs.langmuir.9b03687
Chevreuil, M., Lecoq, L., Wang, S., Gargowitsch, L., Nhiri, N., Jacquet, E., Zinn, T., Fieulaine, S., Bressanelli, S., & Tresset, G. (2020). Nonsymmetrical Dynamics of the HBV Capsid Assembly and Disassembly Evidenced by Their Transient Species. The Journal of Physical Chemistry B, 124(45), 9987–9995. https://doi.org/10.1021/acs.jpcb.0c05024
Tresset, G. (2013). Compartimentation microscopique: depuis kes microchambres femtolitriques jusqu’aux particules pseudo-virales [HDR]. Université Paris-Sud.
Ni, M., Tresset, G., Iliescu, C., & Hauser, C. A. E. (n.d.). Ultrashort Peptide Theranostic Nanoparticles by Microfluidic-Assisted Rapid Solvent Exchange [IEEE].
Ni, M., Tresset, G., Iliescu, C., & Hauser, C. A. E. (2020). Ultrashort Peptide Theranostic Nanoparticles by Microfluidic-Assisted Rapid Solvent Exchange. IEEE Transactions on NanoBioscience, 19(4), 627–632. https://doi.org/10.1109/TNB.2020.3007103
Panahandeh, S., Li, S., Marichal, L., Leite Rubim, R., Tresset, G., & Zandi, R. (2020). How a Virus Circumvents Energy Barriers to Form Symmetric Shells. ACS Nano, 14(3), 3170–3180. https://doi.org/10.1021/acsnano.9b08354
Tresset, G., Castelnovo, M., & Leforestier, A. (2017). Assemblage et désassemblage des virus : mode d’emploi. Reflets de La Physique, 52, 22–26. https://doi.org/10.1051/refdp/201752022
Illy, N., Corcé, V., Zimbron, J., Molinié, V., Labourel, M., Tresset, G., Degrouard, J., Salmain, M., & Guégan, P. (2019). pH‐Sensitive Poly(ethylene glycol)/Poly(ethoxyethyl glycidyl ether) Block Copolymers: Synthesis, Characterization, Encapsulation, and Delivery of a Hydrophobic Drug. Macromolecular Chemistry and Physics, 1900210. https://doi.org/10.1002/macp.201900210
Chevreuil, M., Fieulaine, S., Poncet, L., Perronet, K., Zinn, T., Jacquet, E., Nhiri, N., Bressanelli, S., & Tresset, G. (2019). Characterization of the Assembly and Disassembly of Capsid Proteins Derived from Hepatitis B Virus. Biophysical Journal, 116(3), 159a. https://doi.org/10.1016/j.bpj.2018.11.879
Gomez, J.-P., Tresset, G., Pichon, C., & Midoux, P. (2019). Improved histidinylated lPEI polyplexes for skeletal muscle cells transfection. International Journal of Pharmaceutics, 559, 58–67. https://doi.org/10.1016/j.ijpharm.2019.01.003
Tresset, G. (2019, January). Impact of RNA on the assembly dynamics of icosahedral and tubular viruses. Physical Virology Gordon Research Conference, Ventura (USA).
Chevreuil, M., Law-Hine, D., Chen, J., Bressanelli, S., Combet, S., Constantin, D., Degrouard, J., Möller, J., Zeghal, M., & Tresset, G. (2018). Nonequilibrium Self-Assembly Dynamics of Icosahedral Viral Capsids Packaging Genome. Biophysical Journal, 114(3), 60a. https://doi.org/10.1016/j.bpj.2017.11.378
Burke, A., Chevreuil, M., Paris, A., de La Grange, V., Goldmann, C., Pérez, J., Constantin, D., & Tresset, G. (2018). Nanoparticle-Templated Self-Assembly of Viral Capsids Probed by Time-Resolved Absorbance Spectroscopy and X-Ray Scattering. Physical Review Applied, 10(5), 054065–054075. https://doi.org/10.1103/PhysRevApplied.10.054065
Chen, J., Lansac, Y., & Tresset, G. (2018). Interactions between the Molecular Components of the Cowpea Chlorotic Mottle Virus Investigated by Molecular Dynamics Simulations. The Journal of Physical Chemistry B, 122(41), 9490–9498. https://doi.org/10.1021/acs.jpcb.8b08026
Tresset, G. (2018, Août). Microfluidics-directed self-assembly of DNA-based nanoparticles. International Microfluidics Congress, San Diego (USA).
Chevreuil, M., Law-Hine, D., Chen, J., Bressanelli, S., Combet, S., Constantin, D., Degrouard, J., Möller, J., Zeghal, M., & Tresset, G. (2018). Nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging genome or polyelectrolyte. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-05426-8
Tresset, G., Chen, J., Chevreuil, M., Nhiri, N., Jacquet, E., & Lansac, Y. (2017). Two-Dimensional Phase Transition of Viral Capsid Gives Insights into Subunit Interactions. Physical Review Applied, 7(1), 014005. https://doi.org/10.1103/PhysRevApplied.7.014005
Tresset, G., Marculescu, C., Salonen, A., Ni, M., & Iliescu, C. (2013). Fine Control Over the Size of Surfactant–Polyelectrolyte Nanoparticles by Hydrodynamic Flow Focusing. Analytical Chemistry, 85(12), 5850–5856. https://doi.org/10.1021/ac4006155
Maury, B., Gonçalves, C., Tresset, G., Zeghal, M., Cheradame, H., Guégan, P., Pichon, C., & Midoux, P. (2014). Influence of pDNA availability on transfection efficiency of polyplexes in non-proliferative cells. Biomaterials, 35(22), 5977–5985. https://doi.org/10.1016/j.biomaterials.2014.04.007
Tresset, G., Tatou, M., Le Cœur, C., Zeghal, M., Bailleux, V., Lecchi, A., Brach, K., Klekotko, M., & Porcar, L. (2014). Weighing Polyelectrolytes Packaged in Viruslike Particles. Physical Review Letters, 113(12), 128305. https://doi.org/10.1103/PhysRevLett.113.128305
Tresset, G., Le Coeur, C., Bryche, J.-F., Tatou, M., Zeghal, M., Charpilienne, A., Poncet, D., Constantin, D., & Bressanelli, S. (2013). Norovirus Capsid Proteins Self-Assemble through Biphasic Kinetics via Long-Lived Stave-like Intermediates. Journal of the American Chemical Society, 135(41), 15373–15381. https://doi.org/10.1021/ja403550f
Tresset, G. (2015). The self-assembly of icosahedral capsids probed by X rays and neutrons. Journée Biophysique Université Paris-Saclay, Gif-sur-Yvette, France.
Tresset, G. (2017, juin). Nonequilibrium Dynamics of RNA Packaging in Icosahedral Viral Capsids. ICTP Workshop on Physical Virology, Trieste, Italie.
Tresset, G. (2016, September). Self-assembly/Disassembly Dynamic Pathways of an Icosahedral Viral Capsid. Proc. 4th International Soft Matter Conference, Grenoble, (France).
Tresset, G. (2016, September). Microfluidics-Directed Self-Assembly of DNA-Based Nanoparticles. Proc. 4th International Soft Matter Conference, Grenoble, (France).
Tresset, G. (2015, Février). Assembly and Disassembly Kinetics of Virus-Like Particles Probed by Time-Resolved Small-Angle X-Ray Scattering. Proc. 10th SOLEIL Users’ Meeting, Palaiseau (France).
Tresset, G. (2014, Août). Weighing Polyelectrolytes Packaged in Virus-Like Particles. Proc. Mathematical Virology Conference, York (UK).
Tresset, G. (2013, October). Norovirus capsid proteins self-assemble through biphasic kinetics via long-lived stave-like intermediates. Proc. 23rd Phage/Virus Assembly, Lake Arrowhead (USA).
Iliescu, C., & Tresset, G. (2015). Microfluidics-Driven Strategy for Size-Controlled DNA Compaction by Slow Diffusion through Water Stream. Chemistry of Materials, 27(24), 8193–8197. https://doi.org/10.1021/acs.chemmater.5b04129
Law-Hine, D., Sahoo, A. K., Bailleux, V., Zeghal, M., Prevost, S., Maiti, P. K., Bressanelli, S., Constantin, D., & Tresset, G. (2015). Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates. The Journal of Physical Chemistry Letters, 6(17), 3471–3476. https://doi.org/10.1021/acs.jpclett.5b01478
Chen, J., Chevreuil, M., Combet, S., Lansac, Y., & Tresset, G. (2017). Investigating the thermal dissociation of viral capsid by lattice model. Journal of Physics: Condensed Matter, 29(47), 474001. https://doi.org/10.1088/1361-648X/aa8d88
Tresset, G., & Iliescu, C. (2016). Microfluidics-Directed Self-Assembly of DNA-Based Nanoparticles. INFORMACIJE MIDEM-JOURNAL OF MICROELECTRONICS ELECTRONIC COMPONENTS AND MATERIALS, Vol. 46, No. 4(2016), 183-189.
Ni, M., Tresset, G., & Iliescu, C. (2017). Self-assembled polysulfone nanoparticles using microfluidic chip. Sensors and Actuators B: Chemical, 252, 458–462. https://doi.org/10.1016/j.snb.2017.06.028
Tresset, G. (2016, September). The Assembly and Disassembly Pathways of Viral Nanoshells Probed by X-Ray and Neutron Scattering. CMD26 Physics of Protein Nanoshells, Groningen, (Pays-Bas).
Tresset, G. (2016, October). Microfluidics-Directed Self-Assembly of DNA-Based Nanoparticles. 52nd International Conference on Microelectronics, Devices and Materials, Ankaran, (Slovénie).
Law-Hine, D., Zeghal, M., Bressanelli, S., Constantin, D., & Tresset, G. (2016). Identification of a major intermediate along the self-assembly pathway of an icosahedral viral capsid by using an analytical model of a spherical patch. Soft Matter, 12(32), 6728–6736. https://doi.org/10.1039/C6SM01060A
Tresset, G. (2015, October). Biomimetic assemblies processed by microfluidics. International Semiconductor Conference, Sinaia (Roumanie).
Tresset, G. (2016, Juillet). What can we learn from the disassembly of icosahedral viral capsids? FASEB SRC Virus Structure and Assembly, Steamboat Springs, USA.

Contact

Laboratoire de Physique des Solides, Université Paris-Saclay, CNRS (Orsay, France)

Jan 2021 – present: Deputy director
Oct 2019 – present: CNRS research director
Jul 2019 – Dec 2020: Head of the « Self-Assembled Biological Objects » team
Oct 2008 – Oct 2019: CNRS research associate

Institute of Bioengineering and Nanotechnology, A*STAR (Republic of Singapore)

Jan 2005 – Sep 2008: Research scientist       

Laboratory for Integrated Micro Mechatronic Systems, The University of Tokyo (Japan)

Nov 2002 – Dec 2004: Postdoctoral fellow

Département de Recherches pour la Fusion Contrôlée, CEA (Saint-Paul-lèz-Durance, France)

Oct 1999 – Sep 2002: Doctoral candidate